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KMID : 0379119870150020108
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Korean Journal of Mycology
1987 Volume.15 No. 2 p.108 ~ p.115
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Synthesis of Trehalose by Pleurotus spp.
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Abstract
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Nutritional characteristics and cultural conditions for trehalose synthesis and mycelial yield of Pleurotus sajor-caju and Pleurotus ostreatus 201 in submerged culture were investigated. The results were as follows: Among the carbon sources, glucose was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of glucose was 1%. Among the nitrogen sources, peptone was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of peptone was 0.05%. The optimal concentration of KH₂PO₄, and MgSO₄7 H₂O for trehalose synthesis and mycelial yield was 0.1%, 0.04% and 0.2%, 0.04-0.08%, respectively. The optimal temperature and pH for trehalose synthesis were 25℃ and pH 5.5. but optimal temperature and pH for mycelial yield were 30℃ and pH 5.5. The maximum yield of trehalose was obtained after 10 day cultivation.
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